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當前位置：首頁 > 資料下載 > GBI Labs D12-18說明書

GBI Labs D12-18說明書

發布時間：2018/8/30      點擊次數：1388

GBI Labs / Golden Bridge International（GBI），Inc。成立于1993年，旨在為世界各地的研究，臨床，生物技術和制藥實驗室提供*，經濟，創新的病理學試劑和試劑盒。今天，GBI實驗室繼續開發新產品，并制造高質量的免疫組織化學（IHC）試劑，用于臨床和非臨床研究中癌癥和其他疾病的抗原/蛋白質篩查; 基于抗體的藥物發現：和人類解剖病理學和獸醫病理學中的傳統應用蛋白質/抗體篩選。

GBI Labs*的系列包括病理學輔助試劑，具有辣根過氧化物酶[HRP]和堿性磷酸酶[AP]聚合物技術的檢測試劑盒，以及除傳統小鼠，兔，大鼠和山羊外的多種宿主物種的檢測。我們擁有多的雙染色和三染色試劑盒，其中含有色原，可以使用光學顯微鏡對共定位蛋白表達進行半定量分析。我們的Klear Human試劑盒可在沒有背景的情況下快速檢測人體組織上的人源或人源化抗體。我們為其他物種提供Klear試劑盒，如小鼠，兔子和大鼠。

特征

  • 一步聚合物檢測系統，靈敏度高。不含生物素。

  • 檢測小鼠IgG

  • 孵化15分鐘。

  • 人和兔血清蛋白被吸附。

  • 選擇色原試劑盒：DAB，DAB-Ni，AEC，Permanent Red，Fast-Red，BCIP / NBT，AP-Blue

Polink-1 HRP Detection System for Mouse Antibody (for DAB) (Polymer-HRP detection system, biotin-free, Anti-mouse) Ready-to-use One Step Polymer Detection System

Polink-1小鼠抗體HRP檢測系統


（聚合物HRP檢測系統，無生物素，抗小鼠）


一步法聚合物檢測系統


Storage: 4-8oC

Catalog No. D12-110 110 ml (bulk, w/o chromogen)

D12-60 60ml (bulk, w/o chromogen)

D12-18 18 ml (with DAB, good for 180 slides) D12-6 6 ml (with DAB, good for 50 slides)


預定用途：


目錄號D12110 110毫升（散裝，W/O顯色劑）


D12-60毫升(體積，w/o色原)


d12-18 18毫升(dda，好180張幻燈片)d12-6 6毫升(dda，好50張幻燈片)


Polink-1 HRP小鼠民建聯檢測試劑盒的目的是利用用戶提供的小鼠抗體在人體組織或細胞標本上檢測靶抗原。標本可以冷凍或石蠟包埋的組織， 新制備的單層細胞涂片。


POLIKI-1 1HRP小鼠DAB檢測試劑盒是一種使用聚合辣根過氧化物酶（HRP）的一步聚合物檢測系統。


-鏈接山羊抗鼠IgG，直接檢測與組織結合的初級抗體。該技術具有良好的敏感性和高特異性。因此，這是一種無生物素的系統。 由內源性生物素1引起鏈霉親和素/生物素系統引起的非特異性染色。這是一個一步檢測系統，比傳統的兩步檢測方法要快得多。 生物素化的第二抗體，然后是鏈霉親和素-HRP)。AEC Stai 寧選擇POLIK-1 HRP小鼠為AEC（D15 110、D15 18、D15～6）。


Kit components:


Catalog No.

Product Name

Reagent 1:

Polymer HRP-linked anti-mouse IgG (Ready-to-use)

Reagent 2:

2A: DAB Substrate

2B: Chromogen concentrate

D12-110

Polink-1 HRP Mouse Bulk for DAB kit

110ml

Not provided

D12-60

Polink-1 HRP Mouse Bulk for DAB kit

60ml

Not provided

D12-18

Polink-1 HRP Mouse DAB kit

18ml

30 ml of 2A and 2 ml of 2B

D12-6

Polink-1 HRP Mouse DAB kit

6ml

12 ml of 2A and 1.5 ml of 2B



Reagent

Staining Procedure

Incubation Time (Min.)

1. Peroxidase Blocking Reagent

Supplied by user

a. Incubate slides in peroxidese blocking reagent (Ready-to-use 3% H2O2 solution) for 10 min.

b. Rinse the slide using distilled water.

10

2. HIER Pretreatment:

Refer to antibody data sheet.

a. Heat Induced Epitope Retrieval (HIER) may be required for primary antibody suggested by vendor.

b. Wash with PBS 3 times for 2 minutes each time.

Refer to vendor’s data sheet

3. Pre-Block (Optional) Not provided

a. Add 2 (100 μL) or more drops of 10% Normal Goat Serum to cover the tissue section and Incubate 10 min.

b. Drain or blot off solution. DO NOT RINSE.

10

4. Primary antibody: Supplied by user

Notes: Investigator needs to optimize dilution and incubation times

a. Apply 2 (100 μL) or more drops of primary antibody to cover the tissue completely. Incubate in moist chamber for 30-60 min.

b. Rinse with PBS containing 0.05% Tween-20 3 times for 2 minutes each time.

30-60

5. Reagent 1: HRP

Polymer-anti-Mouse IgG

a. Apply 2 (100 μL) or more drops of HRP Polymer-anti-Mouse IgG to cover tissue

section and Incubate in moist chamber for 10-15 min.

15



建議的議定書：


固定：為了保證染色的質量和獲得可復制的性能，用戶需要提供適當的固定組織和準備好的幻燈片。


組織需要緊緊粘在滑塊上，以避免組織脫落。


石蠟包埋切片在染色前必須先用二甲苯進行脫粘，再用分級的乙醇進行再水化。


細胞涂片應盡量多做單層，以獲得滿意的結果。


研究人員需要優化原抗體的稀釋和孵育時間。


三種對照幻燈片將有助于解釋結果：陽性組織控制，試劑控制(用等型控制試劑處理的幻燈片)和陰性對照。


進行IHC染色：不要讓標本或組織從這一點開始干燥。


議定書說明：


固定時間、組織切片厚度、一抗體稀釋度和孵育時間對結果有顯著影響。調查人員需要考慮所有因素，并在下列情況下確定宜條件： 解釋結果。


組織染色取決于染色前組織的正確處理和處理。不適當的組織準備可能導致假陰性結果或不一致的結果。


不要混合不同批次的試劑。


在染色過程中，不要讓幻燈片在任何時候干燥。

Related Products:



＊POLIK-1 HRP大鼠NIT試劑盒與小鼠原代抗體不交叉反應


*Polink-1 HRP小鼠-NR試劑盒不與大鼠一次抗體發生交叉反應。



有防備的，戒備的，警惕的:


請戴上手套，并采取其他必要的預防措施。


話語( remark的名詞復數 ):


只供研究用。


參考( reference的名詞復數 ):


Bisgaard K，PluizedKP.聚合物結合物在免疫化學中的應用：傳統染色方法與利用聚合物共軛物染色方法的比較研究。21 Intl Cong Intl Acad Pathol和12 World Cong Acad Environ Pathol.布達佩斯，饑餓，1996年10月20-25日。


石澤。Itzkowitz SH，Kim YS.三種免疫過氧化物酶技術檢測結直腸癌組織抗原的比較j Hitochem Cytochem 36：317-322，



GBI Labs 2018年產品列表：


貨號

品名

規格

品牌

D103-110D

Klear Human HRP-Polymer DAB Bulk with DAB Kit

110mL

GBI Labs

D103-18

Klear Human HRP-Polymer DAB Kit

18ml

GBI Labs

D103-6

Klear Human HRP-Polymer DAB Kit

6ml

GBI Labs

D104-18

Klear Human HRP-polymer with AEC Kit

18ml

GBI Labs

D104-6

Klear Human HRP-polymer with AEC Kit

6ml

GBI Labs

D105-18

Klear Human AP-polymer with Fast Red Kit

18ml

GBI Labs

D105-6

Klear Human AP-polymer with Fast Red Kit

6ml

GBI Labs

D106-18

Klear Human AP-polymer with Permanent Red Kit

18ml

GBI Labs

D106-6

Klear Human AP-polymer with Permanent Red Kit

6ml

GBI Labs

D27-110

Polink-2 AP Broad-3 (Ms. Rb. Gt) Bulk Kit

110ml

GBI Labs

D27-18/D27-6

Polink-2 AP Broad-3 (Ms. Rb. Gt) with Permanent Red Kit

18ml/6ml

GBI Labs

D86-18/D86-6

Polink-2 Plus HRP Syrian Hamster with DAB Kit

18ml/6ml

GBI Labs

D86-60

Polink-2 Plus HRP Syrian Hamster for DAB Bulk Kit

60ml

GBI Labs

D22-110/D22-60

Polink-2 HRP Broad for DAB Bulk Kit

110ml/60ml

GBI Labs

D22-18/D22-6

Polink-2 HRP Broad with DAB Kit

18ml/6ml

GBI Labs

D22-60D

Polink-2 HRP Broad with DAB Kit

60mL

GBI Labs

D23-110/D23-60

Polink-2 HRP Broad for AEC Bulk Kit

110ml/60ml

GBI Labs

D23-18/D23-6

Polink-2 HRP Broad with AEC Kit

18ml/6ml

GBI Labs

D24-110

Polink-2 AP Broad Bulk Kit

110ml

GBI Labs

D24-18/D24-6

Polink-2 AP Broad with Permanent Red Kit

18ml/6ml

GBI Labs

D11-110

Polink-1 HRP Broad for DAB Bulk Kit

110ml

GBI Labs

D11-18/D11-6

Polink-1 HRP Broad with DAB Kit

18ml/6ml

GBI Labs

D11-60

Polink-1 HRP Broad for DAB Bulk Kit

60mL

GBI Labs

D14-110

Polink-1 HRP Broad for AEC Bulk Kit

110ml

GBI Labs

D14-18/D14-6

Polink-1 HRP Broad with AEC Kit

18ml/6ml

GBI Labs

D14-60

Polink-1 HRP Broad for AEC Bulk Kit

60mL

GBI Labs

D17-110

Polink-1 AP Broad Bulk Kit

110ml

GBI Labs

D17-18/D17-6

Polink-1 AP Broad with Permanent Red Kit

18ml/6ml

GBI Labs

D41-110

Polink-2 Plus HRP Broad for DAB Bulk Kit

110ml

GBI Labs

D41-125

Polink-2 Plus HRP Broad with DAB Bulk Kit

125ml

GBI Labs

D41-18/D41-6

Polink-2 Plus HRP Broad with DAB Kit

18ml/6ml

GBI Labs

D42-110

Polink-2 Plus HRP Broad for AEC Bulk Kit

110ml

GBI Labs

D42-18/D42-6

Polink-2 Plus HRP Broad with AEC Kit

18ml/6ml

GBI Labs

D68-110

Polink-2 Plus AP Broad Bulk Kit

110ml

GBI Labs

D68-18/D68-6

Polink-2 Plus AP Broad Kit

18ml/6ml

GBI Labs

D13-18/D13-6

Polink-1 HRP Rabbit with DAB Kit

18ml/6ml

GBI Labs

D16-110

Polink-1 HRP Rabbit for AEC Bulk Kit

110ml

GBI Labs

D16-18/D16-6

Polink-1 HRP Rabbit with AEC Kit

18ml/6ml

GBI Labs

D19-110

Polink-1 AP Rabbit Bulk Kit

110ml

GBI Labs

D19-18/D19-6

Polink-1 AP Rabbit with Permanent Red Kit

18ml/6ml

GBI Labs

D39-110

Polink-2 Plus HRP Rabbit for DAB Bulk Kit

110ml

GBI Labs

D39-18/D39-6

Polink-2 Plus HRP Rabbit with DAB Kit

18ml/6ml

GBI Labs

D40-110

Polink-2 Plus HRP Rabbit for AEC Bulk Kit

110ml

GBI Labs

D40-18/D40-6

Polink-2 Plus HRP Rabbit with AEC Kit

18ml/6ml

GBI Labs

D70-110

Polink-2 Plus AP Rabbit Bulk Kit

110ml

GBI Labs

D70-18/D70-6

Polink-2 Plus AP Rabbit with Permanent Red Kit

18ml/6ml

GBI Labs

D03(2)-110

Biotin-2nd Ab Rabbit

110mL

GBI Labs

D03(2)-18

Biotin-2nd Ab Rabbit

18mL

GBI Labs

D03-110

SPlink HRP Rabbit Bulk Kit

110ml

GBI Labs

D03-18/D03-6

SPlink HRP Rabbit with DAB Kit

18ml/6ml

GBI Labs

D03-500

SPlink HRP Rabbit Bulk Kit

500mL

GBI Labs

D34-18/D34-6

Polink-1 HRP Goat with AEC Kit

18ml/6ml

GBI Labs

D61-110

Polink-1 AP Goat Bulk Kit

110ml

GBI Labs

D61-18/D61-6

Polink-1 AP Goat with Permanent Red Kit

18ml/6ml

GBI Labs

D43-110

Polink-2 Plus HRP Goat for DAB Bulk Kit

110ml

GBI Labs

D43-18/D43-6

Polink-2 Plus HRP Goat with DAB Kit

18ml/6ml

GBI Labs

D45-110

Polink-2 Plus HRP Goat for AEC Bulk Kit

110ml

GBI Labs

D45-18/D45-6

Polink-2 Plus HRP Goat with AEC Kit

18ml/6ml

GBI Labs

D66-110

Polink-2 Plus AP Goat Bulk Kit

110ml

GBI Labs

D66-18/D66-6

Polink-2 Plus AP Goat with Permanent Red Kit

18ml/6ml

GBI Labs

D76(2)-18

Biotin-2nd Ab Goat

18mL

GBI Labs

D76-110

SPlink HRP Goat Bulk Kit

110ml

GBI Labs

D76-18/D76-6

SPlink HRP Goat with DAB Kit

18ml/6ml

GBI Labs

D77-18/D77-6

SPlink HRP Goat AEC Kit

18ml/6ml

GBI Labs

D84-18/D84-6

Polink-2 Plus HRP Chicken IgY with DAB Kit

18ml/6ml

GBI Labs

D84-60

Polink-2 Plus HRP Chicken IgY for DAB Kit

60ml

GBI Labs

D92-18/D92-6

Splink HRP Chicken IgY with DAB Kit

18ml/6ml

GBI Labs

D92-60

Splink HRP Chicken IgY Bulk Kit

60ml

GBI Labs

D93-18/D93-6

Splink HRP Chicken IgY with AEC Kit

18ml/6ml

GBI Labs

D85-18/D85-6

Polink-2 Plus HRP Sheep with DAB Kit

18ml/6ml

GBI Labs

D52-18/D52-6

Klear Mouse HRP with DAB Kit

18ml/6ml

GBI Labs

D53-110

Klear Mouse HRP for AEC Bulk Kit

110ml

GBI Labs

D53-18/D53-6

Klear Mouse HRP with AEC Kit

18ml/6ml

GBI Labs

D54-100

Klear Mouse Blocking Buffer

100mL

GBI Labs

D54-18/D54-6

Klear Mouse Blocking Buffer

18mL/6mL

GBI Labs

D55-110

Polink-1 HRP Mouse-NR for DAB Bulk Kit

110ml

GBI Labs

D55-18/D55-6

Polink-1 HRP Mouse-NR with DAB Kit

18ml/6ml

GBI Labs

D34-110

Polink-1 HRP Goat for AEC Bulk Kit

110ml

GBI Labs

D34-18/D34-6

Polink-1 HRP Goat with AEC Kit

18ml/6ml

GBI Labs

D61-110

Polink-1 AP Goat Bulk Kit

110ml

GBI Labs

D61-18/D61-6

Polink-1 AP Goat with Permanent Red Kit

18ml/6ml

GBI Labs

D43-110

Polink-2 Plus HRP Goat for DAB Bulk Kit

110ml

GBI Labs

D43-18/D43-6

Polink-2 Plus HRP Goat with DAB Kit

18ml/6ml

GBI Labs

D45-110

Polink-2 Plus HRP Goat for AEC Bulk Kit

110ml

GBI Labs

D45-18/D45-6

Polink-2 Plus HRP Goat with AEC Kit

18ml/6ml

GBI Labs

D66-110

Polink-2 Plus AP Goat Bulk Kit

110ml

GBI Labs

D66-18/D66-6

Polink-2 Plus AP Goat with Permanent Red Kit

18ml/6ml

GBI Labs

D76-110

SPlink HRP Goat for DAB Bulk Kit

110ml

GBI Labs

D76-18/D76-6

SPlink HRP Goat with DAB Kit

18ml/6ml

GBI Labs

D77-18/D77-6

SPlink HRP Goat with AEC Kit

18ml/6ml

GBI Labs

D84-18/D84-6

Polink-2 Plus HRP Chicken IgY with DAB Kit

18ml/6ml

GBI Labs

DS201B-6 (D63-6)

Polink DS-MR-Hu B1 Kit

12ml

GBI Labs

DS201B-60

Polink DS-MR-Hu B1 Kit

120ml

GBI Labs

DS201C-18

Polink DS-MR-Hu C1 Kit

36mL

GBI Labs

DS201C-6

Polink DS-MR-Hu C1 Kit

12mL

GBI Labs

DS201C-60

Polink DS-MR-Hu C1 Kit

120mL

GBI Labs

DS201D-18

Polink DS-MR-Hu D1 Kit

36ml

GBI Labs

DS201D-6 (D20-6F)

Polink DS-MR-Hu D1 Kit

12ml

GBI Labs

DS201D-60

Polink DS-MR-Hu D1 Kit

120ml

GBI Labs

DS202A-18

Polink DS-MR-Hu A2 Kit

36mL

GBI Labs

DS202A-6 (D32-6A)

Polink DS-MR-Hu A2 Kit

12mL

GBI Labs

DS202A-60

Polink DS-MR-Hu A2 Kit

120mL

GBI Labs

DS202B-18

Polink DS-MR-Hu B2 Kit

36ml

GBI Labs

DS202B-6 (D64-6)

Polink DS-MR-Hu B2 Kit

12ml

GBI Labs

DS202B-60

Polink DS-MR-Hu B2 Kit

120ml

GBI Labs

DS202C-18

Polink DS-MR-Hu C2 Kit

36ml

GBI Labs

DS202C-6

Polink DS-MR-Hu C2 Kit

12ml

GBI Labs

DS202C-60

Polink DS-MR-Hu C2 Kit

120ml

GBI Labs

DS202D-18

Polink DS-MR-Hu D2 Kit

36ml

GBI Labs

DS202D-6 (D32-6F)

Polink DS-MR-Hu D2 Kit

12ml

GBI Labs

DS202D-60

Polink DS-MR-Hu D2 Kit

120ml

GBI Labs

DS203A-18

Polink DS-MM-Hu A Kit

36mL

GBI Labs

DS203A-6 (D78-6A)

Polink DS-MM-Hu A Kit

12mL

GBI Labs

DS203A-60

Polink DS-MM-Hu A Kit

120mL

GBI Labs

DS203B-18

Polink DS-MM-Hu B Kit

36ml

GBI Labs

DS203B-6 (D79-6)

Polink DS-MM-Hu B Kit

12ml

GBI Labs

DS203B-60

Polink DS-MM-Hu B Kit

120ml

GBI Labs

DS203C-18

Polink DS-MM-Hu C Kit

36mL

GBI Labs

DS203C-6

Polink DS-MM-Hu C Kit

12mL

GBI Labs

DS203C-60

Polink DS-MM-Hu C Kit

120mL

GBI Labs

DS203D-18

Polink DS-MM-Hu D Kit

36ml

GBI Labs

DS203D-6 (D78-6F)

Polink DS-MM-Hu D Kit

12ml

GBI Labs

DS203D-60

Polink DS-MM-Hu D Kit

120ml

GBI Labs

DS204A-18

Polink DS-RR-Hu/Ms A Kit

36ml

GBI Labs

DS204A-6 (D80-6A)

Polink DS-RR-Hu/Ms A Kit

12ml

GBI Labs

DS204A-60

Polink DS-RR-Hu/Ms A Kit

120ml

GBI Labs

DS204B-18

Polink DS-RR-Hu/Ms B Kit

36ml

GBI Labs

DS204B-6 (D81-6)

Polink DS-RR-Hu/Ms B Kit

12ml

GBI Labs

DS204B-60

Polink DS-RR-Hu/Ms B Kit

120ml

GBI Labs

DS204C-18

Polink DS-RR-Hu/Ms C Kit

36mL

GBI Labs

DS204C-6

Polink DS-RR-Hu/Ms C Kit

12mL

GBI Labs

DS204C-60

Polink DS-RR-Hu/Ms C Kit

120mL

GBI Labs

DS204D-18

Polink DS-RR-Hu/Ms D Kit

36ml

GBI Labs

DS204D-6 (D80-6F)

Polink DS-RR-Hu/Ms D Kit

12ml

GBI Labs

DS204D-60

Polink DS-RR-Hu/Ms D Kit

120ml

GBI Labs

DS207A-18

Polink DS-GM-Hu A Kit

36ml

GBI Labs

DS207A-6

Polink DS-GM-Hu A Kit

12ml

GBI Labs

DS207A-60

Polink DS-GM-Hu A Kit

120ml

GBI Labs

DS207B-18

Polink DS-GM-Hu B Kit

36ml

GBI Labs

DS207B-6

Polink DS-GM-Hu B Kit

12ml

GBI Labs

DS207B-60

Polink DS-GM-Hu B Kit

120ml

GBI Labs

DS207C-18

Polink DS-GM-Hu C Kit

36ml

GBI Labs

DS207C-6

Polink DS-GM-Hu C Kit

12ml

GBI Labs

DS207C-60

Polink DS-GM-Hu C Kit

120ml

GBI Labs



上海起發實驗試劑有限公司是實驗試劑一站式采購服務商

1：*的進口輻射能力，血清、抗體、耗材、大部分限制進口品等。

2：產品種類齊全，經營超過700多品牌，基本涵蓋所有生物實驗試劑耗材。

3：提供加急服務，貨品一般1-2周到貨。

4：富有競爭力的價格優勢，絕大部分價格有優勢。

5：多年積累良好的信譽，大部分客戶提供貨到付款服務?？蛻舭ㄇ迦A、北大、交大、復旦、中山等100多所高校，ROCHE，阿斯利康、國藥、fisher等知藥企。

6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權代理。

7：我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批發，歡迎合作。





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