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15921799099
GBI Labs / Golden Bridge International（GBI），Inc。成立于1993年，旨在為世界各地的研究，臨床，生物技術和制藥實驗室提供*，經濟，創新的病理學試劑和試劑盒。今天，GBI實驗室繼續開發新產品，并制造高質量的免疫組織化學（IHC）試劑，用于臨床和非臨床研究中癌癥和其他疾病的抗原/蛋白質篩查; 基于抗體的藥物發現：和人類解剖病理學和獸醫病理學中的傳統應用蛋白質/抗體篩選。
GBI Labs*的系列包括病理學輔助試劑，具有辣根過氧化物酶[HRP]和堿性磷酸酶[AP]聚合物技術的檢測試劑盒，以及除傳統小鼠，兔，大鼠和山羊外的多種宿主物種的檢測。我們擁有多的雙染色和三染色試劑盒，其中含有色原，可以使用光學顯微鏡對共定位蛋白表達進行半定量分析。我們的Klear Human試劑盒可在沒有背景的情況下快速檢測人體組織上的人源或人源化抗體。我們為其他物種提供Klear試劑盒，如小鼠，兔子和大鼠。
特征
專門設計用于檢測人體組織上的人IgG抗體，背景干凈。
該聚合物檢測系統吸附于人血清蛋白，以獲得優異的特異性和靈敏度。
一如既往，GBI聚合物試劑盒不含生物素，可節省額外的阻斷步驟。
簡單的快速協議
將引物和人一抗在4 ℃ 溫育過夜。
將人1'Ab混合物在組織上孵育（30分鐘至2小時）
將抗人聚合物-HRP綴合物孵育10分鐘。
5至20分鐘的色原培養。選擇色原試劑盒：DAB，DAB-Ni，AEC，Permanent Red，Fast-Red，BCIP / NBT，AP-Blue。
Klear Human HRP-Polymer DAB Detection System
核人HRP-聚合物民建聯檢測系統
預定用途：
采用間接檢測法時，用同一種屬的一次抗體進行抗原檢測，可產生較高的背景值。這嚴重限制了篩查人抗體的使用。 在人體組織上。GBI實驗室KlearHumanHRP用于民建聯-聚合物檢測系統設計，用于在人體組織上無背景染色的人原抗體染色。核人HRP-聚合物 檢測試劑盒提供特殊的阻斷緩沖液，聚合HRP連接的二次抗體以及人的引物在一個現成的使用系統。這項技術需要與pri進行通宵的預孵化。 瑪麗抗體具有良好的敏感性和高特異性。它是一種不含生物素的系統，克服了由鏈霉親和素/生物素系統引起的非特異性染色。 奧斯生物素。注：本試劑盒適用于細胞質和膜結合抗原，其他染色模式尚未檢測。
組件
Component No. | Content | 6mL Kit | 18mL Kit | 110mL Kit |
Reagent 1 | Human Primer (RTU) | 6mL | 18mL | 110 mL |
Reagent 2 | Quenching Buffer (5xConcentrate) | 1.5mL | 2.3mLx2 | 13 mL x2 |
Reagent 3 | Hu Blocking A (RTU) | 6mL | 18mL | 110 mL |
Reagent 4 | Hu Blocking B (RTU) | 6mL | 18mL | 110 mL |
Reagent 5 | Human HRP Polymer (RTU) | 6mL | 18mL | 110 mL |
Reagent 6A | DAB Substrate (RTU) | 12mL | 2x15mL | 120 mL |
Reagent 6B | DAB Chromogen (20x) | 1.5mL | 2mL | 6 mL |
建議的議定書：
固定：為了保證染色的質量和獲得可復制的性能，用戶需要提供適當的固定組織和準備好的幻燈片。
組織需要緊緊粘在滑塊上，以避免組織脫落。
石蠟包埋切片在染色前必須先用二甲苯進行脫粘，再用分級的乙醇進行再水化。
細胞涂片應盡量制成單層，以獲得滿意的結果。
三種對照幻燈片將有助于解釋結果：陽性組織控制，試劑控制(用等型對照試劑處理)，和陰性對照。
開始染色程序：不要讓標本或組織從這一點開始干燥。
議定書說明：
固定、組織切片厚度、抗原提取、一次抗體稀釋和孵育時間效應均有顯著性差異(P<0.05)。調查人員需要考慮所有因素并確定宜方案。 解釋結果時的條件。
組織染色取決于染色前組織的正確處理和處理。不適當的組織準備可能導致假陰性結果或不一致的結果。
不要混合不同批次的試劑。
在染色過程中，不要讓幻燈片在任何時候干燥。
相關產品：
Product | Catalog No. | Size |
Klear Mouse AP Fast Red kit | D50-6 / D50-18 | 6ml / 18ml |
Klear Mouse HRP with AEC Kit | D53-6 / D53-18 | 6ml / 18ml |
Klear Mouse AP AP-Red Kit | D51-6 / D51-18 | 6ml / 18ml |
Klear Mouse Blocking A & B | D54-100 / D54-18 | 100ml / 18ml |
Klear Rat HRP DAB kit | D98-6 / D98-18 | 6ml / 18ml |
Klear Rat HRP AEC kit | D99-6 / D99-18 | 6ml / 18ml |
Klear Rat AP Fast-Red | D100-6 / D100-18 | 6ml / 18ml |
Klear Rat AP AP-red | D101-6 / D101-18 | 6ml / 18ml |
Klear Rat Blocking A & B | D102-18 | 18ml |
Polink-2 Plus HRP RAT-NM DAB kit for Rat antibody on Mouse Tissue | D46-6 / D46-18 | 6ml / 18ml |
Polink-2 Plus HRP RAT-NM AEC kit for Rat antibody on Mouse Tissue | D48-6 / D48-18 | 6ml / 18ml |
Polink-2 Plus AP RAT-NM kit for Rat antibody on Mouse Tissue | D67-18 / D67-6 | 6ml / 18ml |
Polink-2 Plus HRP Mouse-NR DAB kit for Mouse antibody on Rat tissue | D58-6 / D58-18 | 6ml / 18ml |
Polink-2 Plus HRP Mouse-NR AEC kit for Mouse antibody on Rat tissue | D59-6 / D59-18 | 6ml / 18ml |
Polink-2 Plus AP Mouse-NR kit for Mouse antibody on Rat tissue | D65-18 / D65-6 | 6ml / 18ml |
警惕
當處理任何或所有試劑時，應將所有組件作為潛在危險材料處理，請戴手套、保護眼睛、適當的實驗室整體(除實驗室外套外)。
話語( remark的名詞復數 ):
只作研究用途
貨號 | 品名 | 規格 | |
D103-110D | Klear Human HRP-Polymer DAB Bulk with DAB Kit | 110mL | GBI Labs |
D103-18 | Klear Human HRP-Polymer DAB Kit | 18ml | GBI Labs |
D103-6 | Klear Human HRP-Polymer DAB Kit | 6ml | GBI Labs |
D104-18 | Klear Human HRP-polymer with AEC Kit | 18ml | GBI Labs |
D104-6 | Klear Human HRP-polymer with AEC Kit | 6ml | GBI Labs |
D105-18 | Klear Human AP-polymer with Fast Red Kit | 18ml | GBI Labs |
D105-6 | Klear Human AP-polymer with Fast Red Kit | 6ml | GBI Labs |
D106-18 | Klear Human AP-polymer with Permanent Red Kit | 18ml | GBI Labs |
D106-6 | Klear Human AP-polymer with Permanent Red Kit | 6ml | GBI Labs |
D27-110 | Polink-2 AP Broad-3 (Ms. Rb. Gt) Bulk Kit | 110ml | GBI Labs |
D27-18/D27-6 | Polink-2 AP Broad-3 (Ms. Rb. Gt) with Permanent Red Kit | 18ml/6ml | GBI Labs |
D86-18/D86-6 | Polink-2 Plus HRP Syrian Hamster with DAB Kit | 18ml/6ml | GBI Labs |
D86-60 | Polink-2 Plus HRP Syrian Hamster for DAB Bulk Kit | 60ml | GBI Labs |
D22-110/D22-60 | Polink-2 HRP Broad for DAB Bulk Kit | 110ml/60ml | GBI Labs |
D22-18/D22-6 | Polink-2 HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D22-60D | Polink-2 HRP Broad with DAB Kit | 60mL | GBI Labs |
D23-110/D23-60 | Polink-2 HRP Broad for AEC Bulk Kit | 110ml/60ml | GBI Labs |
D23-18/D23-6 | Polink-2 HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D24-110 | Polink-2 AP Broad Bulk Kit | 110ml | GBI Labs |
D24-18/D24-6 | Polink-2 AP Broad with Permanent Red Kit | 18ml/6ml | GBI Labs |
D11-110 | Polink-1 HRP Broad for DAB Bulk Kit | 110ml | GBI Labs |
D11-18/D11-6 | Polink-1 HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D11-60 | Polink-1 HRP Broad for DAB Bulk Kit | 60mL | GBI Labs |
D14-110 | Polink-1 HRP Broad for AEC Bulk Kit | 110ml | GBI Labs |
D14-18/D14-6 | Polink-1 HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D14-60 | Polink-1 HRP Broad for AEC Bulk Kit | 60mL | GBI Labs |
D17-110 | Polink-1 AP Broad Bulk Kit | 110ml | GBI Labs |
D17-18/D17-6 | Polink-1 AP Broad with Permanent Red Kit | 18ml/6ml | GBI Labs |
D41-110 | Polink-2 Plus HRP Broad for DAB Bulk Kit | 110ml | GBI Labs |
D41-125 | Polink-2 Plus HRP Broad with DAB Bulk Kit | 125ml | GBI Labs |
D41-18/D41-6 | Polink-2 Plus HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D42-110 | Polink-2 Plus HRP Broad for AEC Bulk Kit | 110ml | GBI Labs |
D42-18/D42-6 | Polink-2 Plus HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D68-110 | Polink-2 Plus AP Broad Bulk Kit | 110ml | GBI Labs |
D68-18/D68-6 | Polink-2 Plus AP Broad Kit | 18ml/6ml | GBI Labs |
D01(2)-110/D01(2)-18 | Biotin-2nd Ab Mouse & Rabbit | 110mL/18mL | GBI Labs |
D01-110/D01-60 | SPlink HRP Broad Bulk Kit | 110ml/60ml | GBI Labs |
D01-125 | SPlink HRP Broad Bulk Kit | 125mL | GBI Labs |
D01-18/D01-6 | SPlink HRP Broad with DAB Kit | 18ml/6ml | GBI Labs |
D04-18/D04-6 | SPlink HRP Broad with AEC Kit | 18ml/6ml | GBI Labs |
D07-110/ D07-60 | APlink AP Broad Bulk Kit | 110ml/60ml | GBI Labs |
D07-18A/D07-6A | APlink AP Broad with AP-Red+ Kit | 18ml/6ml | GBI Labs |
D07-18F/D07-6F | APlink AP Broad with Fast Red Kit | 18ml/6ml | GBI Labs |
D25-18 | Streptavidin-HRP 18ml kit (no 2nd antibody provided) | 18ml | GBI Labs |
D25-500/D25-110 | Streptavidin-HRP Bulk Kit (no 2nd antibody provided) | 500ml/110ml | GBI Labs |
D29-110 | Streptavidin-AP Bulk Kit (no 2nd antibody provided) | 110ml | GBI Labs |
D29-18 | Streptavidin-AP 18ml kit (no 2nd antibody provided) | 18ml | GBI Labs |
D12-110 | Polink-1 HRP Mouse for DAB Bulk Kit | 110ml | GBI Labs |
D12-18/D12-6 | Polink-1 HRP Mouse with DAB Kit | 18ml/6ml | GBI Labs |
D12-60 | Polink-1 HRP Mouse for DAB Bulk Kit | 60mL | GBI Labs |
D12-60D | Polink-1 HRP Mouse for DAB Bulk Kit | 60mL | GBI Labs |
D15-110 | Polink-1 HRP Mouse for AEC Bulk Kit | 110ml | GBI Labs |
D15-18/D15-6 | Polink-1 HRP Mouse with AEC Kit | 18ml/6ml | GBI Labs |
D18-110 | Polink-1 AP Mouse Bulk Kit | 110ml | GBI Labs |
D18-18/D18-6 | Polink-1 AP Mouse with Permanent Red Kit | 18ml/6ml | GBI Labs |
D37-110 | Polink-2 Plus HRP Mouse for DAB Bulk Kit | 110ml | GBI Labs |
D37-18/D37-6 | Polink-2 Plus HRP Mouse with DAB Kit | 18ml/6ml | GBI Labs |
D38-110 | Polink-2 Plus HRP Mouse for AEC Bulk Kit | 110ml | GBI Labs |
D38-18/D38-6 | Polink-2 Plus HRP Mouse with AEC Kit | 18ml/6ml | GBI Labs |
D69-110 | Polink-2 Plus AP Mouse Bulk Kit | 110ml | GBI Labs |
D69-18/D69-6 | Polink-2 Plus AP Mouse with Permanent Red Kit | 18ml/6ml | GBI Labs |
D02(2)-110 | Biotin-2nd Ab Mouse | 110mL | GBI Labs |
D02(2)-18 | Biotin-2nd Ab Mouse | 18mL | GBI Labs |
D02-110 | SPlink HRP Mouse Bulk Kit | 110ml | GBI Labs |
D02-18/D02-6 | SPlink HRP Mouse with DAB Kit | 18ml/6ml | GBI Labs |
D02-500 | SPlink HRP Mouse Bulk Kit | 500mL | GBI Labs |
D05-18/D05-6 | SPlink HRP Mouse with AEC Kit | 18ml/6ml | GBI Labs |
D08-110 | APlink AP Mouse Bulk Kit | 110ml | GBI Labs |
D08-18A/D08-6A | APlink AP Mouse with Permanent Red Kit | 18ml/6ml | GBI Labs |
D08-18F/D08-6F | APllink AP Mouse with Fast Red Kit | 18ml/6ml | GBI Labs |
D31-1 | Simplified Streptavidin-HRP Mouse Concentrate (100x) | 1ml | GBI Labs |
D13-110 | Polink-1 HRP Rabbit for DAB Bulk Kit | 110ml | GBI Labs |
D13-18/D13-6 | Polink-1 HRP Rabbit with DAB Kit | 18ml/6ml | GBI Labs |
D16-110 | Polink-1 HRP Rabbit for AEC Bulk Kit | 110ml | GBI Labs |
D16-18/D16-6 | Polink-1 HRP Rabbit with AEC Kit | 18ml/6ml | GBI Labs |
D19-110 | Polink-1 AP Rabbit Bulk Kit | 110ml | GBI Labs |
D19-18/D19-6 | Polink-1 AP Rabbit with Permanent Red Kit | 18ml/6ml | GBI Labs |
D39-110 | Polink-2 Plus HRP Rabbit for DAB Bulk Kit | 110ml | GBI Labs |
D39-18/D39-6 | Polink-2 Plus HRP Rabbit with DAB Kit | 18ml/6ml | GBI Labs |
D40-110 | Polink-2 Plus HRP Rabbit for AEC Bulk Kit | 110ml | GBI Labs |
D40-18/D40-6 | Polink-2 Plus HRP Rabbit with AEC Kit | 18ml/6ml | GBI Labs |
D70-110 | Polink-2 Plus AP Rabbit Bulk Kit | 110ml | GBI Labs |
D70-18/D70-6 | Polink-2 Plus AP Rabbit with Permanent Red Kit | 18ml/6ml | GBI Labs |
D03(2)-110 | Biotin-2nd Ab Rabbit | 110mL | GBI Labs |
D03(2)-18 | Biotin-2nd Ab Rabbit | 18mL | GBI Labs |
D03-110 | SPlink HRP Rabbit Bulk Kit | 110ml | GBI Labs |
D03-18/D03-6 | SPlink HRP Rabbit with DAB Kit | 18ml/6ml | GBI Labs |
D51-18/D51-6 | Klear Mouse AP with Permanent Red Kit | 18ml/6ml | GBI Labs |
D52-110 | Klear Mouse HRP for DAB Bulk Kit | 110ml | GBI Labs |
D52-18/D52-6 | Klear Mouse HRP with DAB Kit | 18ml/6ml | GBI Labs |
D53-110 | Klear Mouse HRP for AEC Bulk Kit | 110ml | GBI Labs |
D53-18/D53-6 | Klear Mouse HRP with AEC Kit | 18ml/6ml | GBI Labs |
D54-100 | Klear Mouse Blocking Buffer | 100mL | GBI Labs |
D54-18/D54-6 | Klear Mouse Blocking Buffer | 18mL/6mL | GBI Labs |
D55-110 | Polink-1 HRP Mouse-NR for DAB Bulk Kit | 110ml | GBI Labs |
D55-18/D55-6 | Polink-1 HRP Mouse-NR with DAB Kit | 18ml/6ml | GBI Labs |
D56-110 | Polink-1 HRP Mouse-NR for AEC Bulk Kit | 110ml | GBI Labs |
D56-18/D56-6 | Polink-1 HRP Mouse-NR with AEC Kit | 18ml/6ml | GBI Labs |
D57-110 | Polink-1 AP Mouse-NR Bulk Kit | 110ml | GBI Labs |
D57-18/D57-6 | Polink-1 AP Mouse-NR Kit | 18ml/6ml | GBI Labs |
D58-110 | Polink-2 Plus HRP Mouse-NR for DAB Bulk Kit | 110ml | GBI Labs |
D58-18/D58-6 | Polink-2 Plus HRP Mouse-NR with DAB Kit | 18ml/6ml | GBI Labs |
D59-110 | Polink-2 Plus HRP Mouse-NR for AEC Bulk Kit | 110ml | GBI Labs |
D59-18 /D59-6 | Polink-2 Plus HRP Mouse-NR with AEC Kit | 18ml/6ml | GBI Labs |
D65-110 | Polink-2 Plus AP Mouse-NR Bulk Kit | 110ml | GBI Labs |
D65-18/D65-6 | Polink-2 Plus AP Mouse-NR Kit | 18ml/6ml | GBI Labs |
D12-110 | Polink-1 HRP Mouse for DAB Bulk Kit | 110ml | GBI Labs |
D12-18/D12-6 | Polink-1 HRP Mouse with DAB Kit | 18ml/6ml | GBI Labs |
D15-110 | Polink-1 HRP Mouse for AEC Bulk Kit | 110ml | GBI Labs |
D15-18/D15-6 | Polink-1 HRP Mouse with AEC Kit | 18ml/6ml | GBI Labs |
D18-110 | Polink-1 AP Mouse Bulk Kit | 110ml | GBI Labs |
D18-18/D18-6 | Polink-1 AP Mouse with GBI-Permanent Red Kit | 18ml/6ml | GBI Labs |
上海起發實驗試劑有限公司是實驗試劑一站式采購服務商
1：*的進口輻射能力，血清、抗體、耗材、大部分限制進口品等。
2：產品種類齊全，經營超過700多品牌，基本涵蓋所有生物實驗試劑耗材。
3：提供加急服務，貨品一般1-2周到貨。
4：富有競爭力的價格優勢，絕大部分價格有優勢。
5：多年積累良好的信譽，大部分客戶提供貨到付款服務。客戶包括清華、北大、交大、復旦、中山等100多所高校，ROCHE，阿斯利康、國藥、fisher等知藥企。
6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權代理。
7：我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批發，歡迎合作。
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